Metabolomic Analysis Driven Search for Biologically Active Marine Natural Products from the Mixture of Dredge Bycatch
Tomoya AOYAGI *1, Yasumoto OYADOMARI1, Yoichi NAKAO1, 2
1Department of Chemistry and Biochemistry, Graduate School of Advanced Science and Engineering, Waseda University
2Research Institute for Science and Engineering, Waseda University
1. Purpose
Marine invertebrates are recognized as a prolific source of structurally unique and biologically potent natural products, some of which have been developed into pharmaceuticals.1) As a treasure trove of novel bioactive compounds, these organisms continue to attract attention as promising leads for drug discovery.
In this study, we focus on the bycatch of deep-sea marine invertebrates collected by dredging, aiming to discover compounds with biological activities from the neglected small fragments of bycatch.
2. Methods and Results
A mixture of small pieces of dredge bycatch (13 kg, wet weight) collected at Oshima-Shinsone, Kagoshima Prefecture was extracted with methanol. The crude extract was fractionated by solvent partitioning, and subsequently normal-phase or reversed-phase chromatography to afford a total of 20 fractions. These fractions were subjected to electrospray ionization mass spectrometry (ESI-MS) and bioassay for cytotoxicity, revealing that the fraction derived from the chloroform layer (Fr.3-2) showed the strongest activity among the fractions. Fr.3-2 was further purified using silica gel open-column chromatography, followed by reversed-phase high-performance liquid chromatography (RP-HPLC). ESI-MS measurements of the active fractions indicated the presence of brominated compounds. Further purification by RP-HPLC yielded the known bromine-containing compound, aerothionin2), together with other related compounds.
In this presentation, combination of ESI-MS and bioassay-guided isolation and identification of bioactive marine substances will be discussed.
3. References
1) Haque, N.; Parveen, S.; Tang, T.; Wei, J.; Huang, Z. Mar. Drugs 2022, 20, 528.
2) Moddy, K.; Thomson, R. H.; Fattorusso, E.; Minale, L.; Sodano, G. J. C. S. Perkin Ⅰ 1972, 18-24.